Could someone please explain the Gel electrophoresis to me? I watched the edrolo video but I still don't get it , thanks
This picture might help. So basically, you put your DNA mixture into the wells. The blue stuff is the gel. You turn on the power, and since DNA is negatively charged, the DNA will move towards the positive end. The smaller fragments will travel farther in the gel than the longer fragments, so they get sperated by size.
DNA is overall negatively charged so it moves away from the + from the electrical source. Since all DNA isn't the same size, their ability to pass between the tiny holes in the gel varies. Basically, the small fragments have an easier time to go through the holes than the larger ones, the smaller ones will travel farther down the gel than the longer ones. When fragments stop moving, they cause lines due to the dye, which is what you see in the final gel sample.
If you have any questions don't hesitate to ask!