Login | Register

Welcome, Guest. Please login or register.

July 12, 2020, 09:48:18 pm

Author Topic: A Spoonful of Sugar- insanipi's PharmSci journey  (Read 28910 times)

0 Members and 1 Guest are viewing this topic.

insanipi

  • National Moderator
  • ATAR Notes Legend
  • *****
  • Posts: 4101
  • "A Bit of Chaos"
  • Respect: +2790
Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #165 on: March 26, 2020, 11:53:18 pm »
+11
After being in such a huge slump in the past week or so (due to the uncertainty surrounding what the heck will happen to me if uni shuts), I found that I had a super productive day today! :D

Today I:
- Made a second draft of my slides
- Went to bookshop (got Oasis by Katya de Becerra)
- finalised my protein edit excel sheet
- Went for a walk down Princes Park (the park across from uni)
- Finished an audiobook (The Ruin- Dervla McTiernan- a book that is not YA- shock!)
- Froze my cells I've been working with in liquid nitrogen
- Started a reading a new review book for an upcoming blog tour

--

As it stands, I'm still required to be in at uni whenever I need to be in, however, with all the rapid changes Monash has made (including shutting both the library and Monash Connect (as of tomorrow) down at Parkville, alongside no cafeteria (since last week)) in the past 24h, I'm seriously unsure if I'll not be on some sort of leave of absence by the end of next week at this stage! I'm trying my best to stay home and work when I don't have to be at uni though, which is the best I can do in the very touch-and-go situation.
« Last Edit: March 26, 2020, 11:58:23 pm by insanipi »
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) (Drug Delivery, Disposition and Dynamics- focusing on molecular biol and editing of glowy proteins)
Follow my uni journey here!

insanipi

  • National Moderator
  • ATAR Notes Legend
  • *****
  • Posts: 4101
  • "A Bit of Chaos"
  • Respect: +2790
Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #166 on: April 06, 2020, 09:23:44 am »
+9

As it stands, I'm still required to be in at uni whenever I need to be in, however, with all the rapid changes Monash has made (including shutting both the library and Monash Connect (as of tomorrow) down at Parkville, alongside no cafeteria (since last week)) in the past 24h, I'm seriously unsure if I'll not be on some sort of leave of absence by the end of next week at this stage! I'm trying my best to stay home and work when I don't have to be at uni though, which is the best I can do in the very touch-and-go situation.
I spoke too soon- last week was a non-lab week for me, so I stayed home and did my stuff here. Little did I know that I'll be doing that for the next month haha 😅

For the first half of last week, I spent a lot of time just napping honestly- I lost all the motivation to do much :( It took a lot of effort to pull myself back into some sort of rhythm haha. As it stands, uni has finally essentially shut down, so my PhD mentor has told me to hang tight and take time to read the literature- something I basically spent the first three weeks doing haha. I currently don't have any coursework to do on Moodle, so I need to think of a new game plan until I get some- perhaps I'll just take the time to read a book a day instead :D (joking- I can't do that, my guilt would stop me haha)

Until next time, stay safe y'all <3
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) (Drug Delivery, Disposition and Dynamics- focusing on molecular biol and editing of glowy proteins)
Follow my uni journey here!

insanipi

  • National Moderator
  • ATAR Notes Legend
  • *****
  • Posts: 4101
  • "A Bit of Chaos"
  • Respect: +2790
Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #167 on: April 21, 2020, 08:36:23 pm »
+13
Hello hello!

I'm starting to get used to being home all the time, I think! I've stopped napping all the time. I even have my desk tidied (first time all year haha- sitting on my bed doing my uni stuff was doing my back in and making me nap all the time haha), and bookshelves sorted (kinda)- and I'm nearly ready to present my project intro Thursday!

I've also been working on my research proposal- and I'm telling you, editing it is like cutting grass with nail clippers. Super tedious, I'm telling you.

I'm super glad that my project is somewhat still going ahead once I'm allowed back into uni, which is something to look forward to! It'll be hard work though, as one of the constructs I require would've been shipped from the US, but I'll have to make in-house, which takes time- but oh well!

Aside from uni stuff, I've been really digging crime and police procedural audiobooks recently, and I have started watching Witcher- it's an interesting show.

Til next time, stay safe xo
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) (Drug Delivery, Disposition and Dynamics- focusing on molecular biol and editing of glowy proteins)
Follow my uni journey here!

insanipi

  • National Moderator
  • ATAR Notes Legend
  • *****
  • Posts: 4101
  • "A Bit of Chaos"
  • Respect: +2790
Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #168 on: May 09, 2020, 02:16:38 pm »
+12
Just as I've really gotten into the swing of things from home, Monash (well, more specifically my PhD mentor Tom tipped me off Sunday night- and confirmed by the head of MIPS not even 24h later) basically told me to start preparing to come back in for lab in the next few weeks.

Whilst this is exciting, I'm still trying to plan out my experiments. This part isn't going very well because nothing is easy when I have less resources than I would've for what I want (and need) to do haha. 😅 The main problem is finding a pair of proteins that have only a few amino acids difference in a row- which is ideal for editing, whilst having a good fluorescence, based on what Parkville currently has in stock. This has proven to be a nightmare and a half this week!

I'm also having a bit of fun occupying some time doing a couple of online courses that were suggested by Estelle, a really lovely postdoc in my research group. They're actually pretty good for filling up time on some of the days so it doesn't feel like I'm not doing as much as I would've in normal circumstances.

Honestly, I'm excited to be heading back into the labs in the near future, and hopefully my experiment planning actually goes somewhere soon!
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) (Drug Delivery, Disposition and Dynamics- focusing on molecular biol and editing of glowy proteins)
Follow my uni journey here!

insanipi

  • National Moderator
  • ATAR Notes Legend
  • *****
  • Posts: 4101
  • "A Bit of Chaos"
  • Respect: +2790
Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #169 on: May 19, 2020, 10:15:59 am »
+9
Just a quick update whilst the library search function appears to be refusing to work haha.

Honestly, I'm excited to be heading back into the labs in the near future, and hopefully my experiment planning actually goes somewhere soon!
My experiment planning is still not going too well- I had a step in the right direction over the weekend, but this new lead kind of is actually hard to work with (or so says my PhD pal), so I'll just have to wait and see what my supervisor says tomorrow -grits teeth in suspense-

Despite this, I'm still keen not to lose hope, even though this project is super tough- not only is the technique I'll be using really new (the first paper on it was released v late last year), but it's also harder in the terms of we can't just order things from overseas to make life easier.

For now, I'm just going to sit here and read papers in cas e I find some other proteins that may work out- at least that's all I really can do until my meeting haha.

I'm keen to start going back from the 26th- given that I really felt that I haven't been doing much whilst stuck at home oops. :D

Stay safe y'all <3
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) (Drug Delivery, Disposition and Dynamics- focusing on molecular biol and editing of glowy proteins)
Follow my uni journey here!

insanipi

  • National Moderator
  • ATAR Notes Legend
  • *****
  • Posts: 4101
  • "A Bit of Chaos"
  • Respect: +2790
Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #170 on: May 26, 2020, 04:01:17 pm »
+8
A quick update!

(You might want to have the volume a little higher than normal, sorry fam haha)
« Last Edit: May 26, 2020, 04:04:35 pm by insanipi »
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) (Drug Delivery, Disposition and Dynamics- focusing on molecular biol and editing of glowy proteins)
Follow my uni journey here!

insanipi

  • National Moderator
  • ATAR Notes Legend
  • *****
  • Posts: 4101
  • "A Bit of Chaos"
  • Respect: +2790
Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #171 on: June 05, 2020, 10:23:36 am »
+6
Just a quick update!

its a video!

Anyways look at the nice view I get from the floor my lab's on :D
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) (Drug Delivery, Disposition and Dynamics- focusing on molecular biol and editing of glowy proteins)
Follow my uni journey here!

insanipi

  • National Moderator
  • ATAR Notes Legend
  • *****
  • Posts: 4101
  • "A Bit of Chaos"
  • Respect: +2790
Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #172 on: June 17, 2020, 09:06:10 pm »
+7
Things I've learnt in the past 12 days that might encourage others:
- Don't overcomplicate things (especially calculating concentrations (c=mv is my friend) and dilutions, overthinking it is the enemy)
- Always be prepared- plan ahead if you can
- It's always worth making an effort or at least an attempt at something you're trying to figure out at first, before giving up
- Don't be afraid to ask the questions, no matter how dumb you think the question is (chances are that it isn't
- Not to drink coffee on an empty stomach (I had the worst cramps from that this morning, realllly don't recommend)
- Similarly, water is not the enemy
- Breathe, and believe in yourself
- Take it easy on Mondays, go hard on the other four weekdays, if you have a case of the Mondays

I'm busy actually doing things in the lab and preparing my actual real important experiments now and it's scary as heck, and I'm also v tired, so I'm am definitely taking it easy every night, so I don't crash out!
« Last Edit: June 17, 2020, 09:13:45 pm by insanipi »
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) (Drug Delivery, Disposition and Dynamics- focusing on molecular biol and editing of glowy proteins)
Follow my uni journey here!

insanipi

  • National Moderator
  • ATAR Notes Legend
  • *****
  • Posts: 4101
  • "A Bit of Chaos"
  • Respect: +2790
Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #173 on: July 04, 2020, 01:37:30 pm »
+7
Howdy!
I'm tired and my back kinda hurts but at least I have progress with my project!

I started roughly planning out the next couple of months based on what I need to get done aims-wise, and it actually turns out that I'd already achieved the first of my three aims- which is actually pretty bloody brilliant because I was starting to worry that I wasn't doing enough. I'm also about to start working on the second aim of mine next week- which includes learning how to make LNPs (lipid nanoparticles) and LPs (lipopeptides). Hopefully I'll be able to start getting results with the third aim sometime in the next four to six weeks too!

I'm also really getting used to cell culture, having not infected nor killed my practice cells in all the time I've been back! <3 I was recently also asked to teach someone else cell culture and it's already been a tough start to doing that, but I'm sure it'll improve. I'm also excited because I finally have a flask of the cells I'm going to be working with, so I'll probably start freezing down stocks of them this week too!

It's pretty cool with my practice cell line that I've been able to transfect properly so that they go from non-fluorescent to fluoroescent, so that's pretty swish too!

Stay safe y'all :)
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) (Drug Delivery, Disposition and Dynamics- focusing on molecular biol and editing of glowy proteins)
Follow my uni journey here!