A Spoonful of Sugar- insanipi's PharmSci journey

[The Cat In The Hat]

Despite my not understanding practically anything about this stuff, it's really interesting. 

[AngelWings]

THE BASIS OF MY WHOLE PROJECT ACTUALLY WORKS! 😍

I ran flow cytometery on Friday for measuring the fluorescence of my cells and I got around 2% positive! This isn't much, but we know it works- so all my hard work so far has been worth it!

That’s amazing to hear! It’s not often that things actually work, especially something experimental (and harder still when it’s during a pandemic). Congrats on this milestone!

[insanipi]

Great news! Well done - happy for you.

So happy for you <33

💚💚💚

Despite my not understanding practically anything about this stuff, it's really interesting. 

Don't worry, I'm still getting my head around it too, despite being nearly 9 months deep into the project haha

That’s amazing to hear! It’s not often that things actually work, especially something experimental (and harder still when it’s during a pandemic). Congrats on this milestone!

I was genuinely expecting it not to work, but alas, it worked! I still cannot get one of the delivery methods to work though (trialling with plain cells to GFP), no matter what I do- it doesn't work aha

I also got some cool images from the microscopy yesterday, which confirms that seeing what I did on flow cytometry last week wasn't just a fluke!

[insanipi]

A bit of a quick update today! It's been nearly a month, and I haven't really acheived much else aside from getting replicates for the data I already had.

This week already has been a drainer and a half- I had a lab class Monday, and I'm in the middle of preparing for my journal critique assessment tomorrow- which is on top of me just setting (just under) half my assays I had planned- I was meant to get another two started, but a machine that I absolutely need busted itself Tuesday, so I don't know when that'll be fixed because I kinda need to get my stuff done and obviously can't (there's only one of this machine on campus, so that's a yikes and a half if I ever saw one :'))

On the bright side of things- I got some pretty cell images yesterday night, and I now have the right to keep my scrunchie obsession up haha!

That's all for now, toodles! <3

[insanipi]

A bit of a quick update today! It's been nearly a month, and I haven't really acheived much else aside from getting replicates for the data I already had.

This week already has been a drainer and a half- I had a lab class Monday, and I'm in the middle of preparing for my journal critique assessment tomorrow- which is on top of me just setting (just under) half my assays I had planned- I was meant to get another two started, but a machine that I absolutely need busted itself Tuesday, so I don't know when that'll be fixed because I kinda need to get my stuff done and obviously can't (there's only one of this machine on campus, so that's a yikes and a half if I ever saw one :'))

On the bright side of things- I got some pretty cell images yesterday night, and I now have the right to keep my scrunchie obsession up haha!

That's all for now, toodles! <3

Following on from this, last week I had a terrible cell contamination event happen so I couldn't restart my experiments when the broken machine got replaced 🙈

That means that I'm having to squeeze 14 assays into the next fortnight; I hope I can do it, because I have a deadline to meet!

Hoping I don't go too insane from it! 😅

[AngelWings]

Following on from this, last week I had a terrible cell contamination event happen so I couldn't restart my experiments when the broken machine got replaced 🙈

That means that I'm having to squeeze 14 assays into the next fortnight; I hope I can do it, because I have a deadline to meet!

Hoping I don't go too insane from it! 😅

That’s absolutely horrifying! 😱 Wishing you good luck from here on out. You’ll make it, Insanipi!

An anecdote from my own Honours project:

Spoiler

I was about 2/3 of the way through the year and had just made my first real model for my project... only for my supervisor to notice that we’d forgotten to cater for one for the big assumptions we said we’d cater for, so they’d taught me the wrong method and my entire first model was incorrect. I was mortified; that was a month’s worth of work down the drain! Fortunately I was able to create the correct model from scratch with some help from my supervisor and managed to recover the work the following week.

[insanipi]

Following on from this, last week I had a terrible cell contamination event happen so I couldn't restart my experiments when the broken machine got replaced 🙈

That means that I'm having to squeeze 14 assays into the next fortnight; I hope I can do it, because I have a deadline to meet!

Hoping I don't go too insane from it! 😅

So. I had more bad things happen to my labwork in the past fortnight, so I'm still doing lab work. But! I'm working it out time-wise so that I'm also doing the bulk of my thesis before my last experiments have had their run! (See also: my thesis writing thread in Accountability and Motivation)

Despite the setbacks (and the breakdown earlier last week), I'm due to plate the last of my experiments tomorrow (to transfect on Tuesday), ready for analysis on the flow cytometer on Friday 5 November!

This year has been a pain, but it's taught me many important lessons too.

[insanipi]

Howdy!
Been a while since I last updated, but today's my presentation and viva day (I'm up in about 20 mins) and I'm super nervous.

That's all for now ahhhhhh <3

[Evolio]

All the best for your presentation!! You got this!

[katie,rinos]

Hope it went well!! 

[Joseph41]

Sending ~good vibes~. Hope it went/is going/goes well!

[Coolmate]

Goodluck insanipi! I hope it goes well and you will smash it

Coolmate

[insanipi]

All the best for your presentation!! You got this!

Hope it went well!! 

Sending ~good vibes~. Hope it went/is going/goes well!

Goodluck insanipi! I hope it goes well and you will smash it

Coolmate

I think I did okay! It finished earlier than I expected, but I wasn't given too hard a question, honestly (which is weird because this was worth 25% of my year, yikes!)

That's pretty much it for my honours year, I'm not sure when results come out (I've been quoted December some time but no actual date has been communicated), but you betcha I'm going to sit there and not read the email with the results included for many hours after I get it

What's next? Honestly, idk - I just really need a break, but I'll figure it out as I go along maybe? (As if that's not what I did this year anyways )

[AngelWings]

Congrats Insanipi! Enjoy your break - you deserve it! How does it feel now that you’re done?