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March 07, 2021, 04:19:02 am

Author Topic: A Spoonful of Sugar- insanipi's PharmSci journey  (Read 35552 times)

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The Cat In The Hat

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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #180 on: September 07, 2020, 09:17:37 am »
+4
Despite my not understanding practically anything about this stuff, it's really interesting.  :)
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AngelWings

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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #181 on: September 07, 2020, 10:32:34 am »
+5
THE BASIS OF MY WHOLE PROJECT ACTUALLY WORKS! 😍

I ran flow cytometery on Friday for measuring the fluorescence of my cells and I got around 2% positive! This isn't much, but we know it works- so all my hard work so far has been worth it!
Thatís amazing to hear! Itís not often that things actually work, especially something experimental (and harder still when itís during a pandemic). Congrats on this milestone!
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insanipi

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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #182 on: September 08, 2020, 11:51:44 am »
+11
Great news! Well done - happy for you. :)
So happy for you <33
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Despite my not understanding practically anything about this stuff, it's really interesting.  :)
Don't worry, I'm still getting my head around it too, despite being nearly 9 months deep into the project haha :P

Thatís amazing to hear! Itís not often that things actually work, especially something experimental (and harder still when itís during a pandemic). Congrats on this milestone!
I was genuinely expecting it not to work, but alas, it worked! I still cannot get one of the delivery methods to work though (trialling with plain cells to GFP), no matter what I do- it doesn't work aha :P

I also got some cool images from the microscopy yesterday, which confirms that seeing what I did on flow cytometry last week wasn't just a fluke! :)
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insanipi

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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #183 on: October 01, 2020, 11:00:05 am »
+17
A bit of a quick update today! It's been nearly a month, and I haven't really acheived much else aside from getting replicates for the data I already had.

This week already has been a drainer and a half- I had a lab class Monday, and I'm in the middle of preparing for my journal critique assessment tomorrow- which is on top of me just setting (just under) half my assays I had planned- I was meant to get another two started, but a machine that I absolutely need busted itself Tuesday, so I don't know when that'll be fixed because I kinda need to get my stuff done and obviously can't (there's only one of this machine on campus, so that's a yikes and a half if I ever saw one :'))

On the bright side of things- I got some pretty cell images yesterday night, and I now have the right to keep my scrunchie obsession up haha!

That's all for now, toodles! <3
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
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insanipi

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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #184 on: October 12, 2020, 07:35:27 am »
+11
A bit of a quick update today! It's been nearly a month, and I haven't really acheived much else aside from getting replicates for the data I already had.

This week already has been a drainer and a half- I had a lab class Monday, and I'm in the middle of preparing for my journal critique assessment tomorrow- which is on top of me just setting (just under) half my assays I had planned- I was meant to get another two started, but a machine that I absolutely need busted itself Tuesday, so I don't know when that'll be fixed because I kinda need to get my stuff done and obviously can't (there's only one of this machine on campus, so that's a yikes and a half if I ever saw one :'))

On the bright side of things- I got some pretty cell images yesterday night, and I now have the right to keep my scrunchie obsession up haha!

That's all for now, toodles! <3
Following on from this, last week I had a terrible cell contamination event happen so I couldn't restart my experiments when the broken machine got replaced 🙈

That means that I'm having to squeeze 14 assays into the next fortnight; I hope I can do it, because I have a deadline to meet!

Hoping I don't go too insane from it! 😅
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) (Drug Delivery, Disposition and Dynamics- focusing on molecular biol and editing of glowy proteins)
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AngelWings

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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #185 on: October 12, 2020, 10:34:42 am »
+10
Following on from this, last week I had a terrible cell contamination event happen so I couldn't restart my experiments when the broken machine got replaced 🙈

That means that I'm having to squeeze 14 assays into the next fortnight; I hope I can do it, because I have a deadline to meet!

Hoping I don't go too insane from it! 😅
Thatís absolutely horrifying! 😱 Wishing you good luck from here on out. Youíll make it, Insanipi!

An anecdote from my own Honours project:
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I was about 2/3 of the way through the year and had just made my first real model for my project... only for my supervisor to notice that weíd forgotten to cater for one for the big assumptions we said weíd cater for, so theyíd taught me the wrong method and my entire first model was incorrect. I was mortified; that was a monthís worth of work down the drain! Fortunately I was able to create the correct model from scratch with some help from my supervisor and managed to recover the work the following week.
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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #186 on: October 25, 2020, 08:23:40 pm »
+9
Following on from this, last week I had a terrible cell contamination event happen so I couldn't restart my experiments when the broken machine got replaced 🙈

That means that I'm having to squeeze 14 assays into the next fortnight; I hope I can do it, because I have a deadline to meet!

Hoping I don't go too insane from it! 😅
So. I had more bad things happen to my labwork in the past fortnight, so I'm still doing lab work. But! I'm working it out time-wise so that I'm also doing the bulk of my thesis before my last experiments have had their run! (See also: my thesis writing thread in Accountability and Motivation)

Despite the setbacks (and the breakdown earlier last week), I'm due to plate the last of my experiments tomorrow (to transfect on Tuesday), ready for analysis on the flow cytometer on Friday 5 November!

This year has been a pain, but it's taught me many important lessons too.
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insanipi

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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #187 on: November 30, 2020, 10:39:34 am »
+9
Howdy!
Been a while since I last updated, but today's my presentation and viva day (I'm up in about 20 mins) and I'm super nervous.

That's all for now ahhhhhh <3
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
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Evolio

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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #188 on: November 30, 2020, 10:55:38 am »
+6
All the best for your presentation!! You got this!
« Last Edit: November 30, 2020, 10:58:15 am by Evolio »

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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #189 on: November 30, 2020, 01:43:53 pm »
+6
Hope it went well!!  :D
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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #190 on: November 30, 2020, 02:54:16 pm »
+6
Sending ~good vibes~. Hope it went/is going/goes well!

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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #191 on: November 30, 2020, 03:11:05 pm »
+6
Goodluck insanipi! I hope it goes well and you will smash it :D

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insanipi

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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #192 on: November 30, 2020, 10:24:35 pm »
+7
All the best for your presentation!! You got this!
Hope it went well!!  :D
Sending ~good vibes~. Hope it went/is going/goes well!
Goodluck insanipi! I hope it goes well and you will smash it :D

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I think I did okay! It finished earlier than I expected, but I wasn't given too hard a question, honestly (which is weird because this was worth 25% of my year, yikes!)

That's pretty much it for my honours year, I'm not sure when results come out (I've been quoted December some time but no actual date has been communicated), but you betcha I'm going to sit there and not read the email with the results included for many hours after I get it :P

What's next? Honestly, idk - I just really need a break, but I'll figure it out as I go along maybe? (As if that's not what I did this year anyways :P )
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) (Drug Delivery, Disposition and Dynamics- focusing on molecular biol and editing of glowy proteins)
Read my uni journey here!
2021: ?

AngelWings

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Re: A Spoonful of Sugar- insanipi's PharmSci journey
« Reply #193 on: December 01, 2020, 11:47:13 am »
+7
Congrats Insanipi! Enjoy your break - you deserve it! How does it feel now that youíre done?
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