Welcome, Guest. Please login or register.

October 18, 2019, 12:04:14 pm

Author Topic: VCE Biology Question Thread  (Read 1380885 times)  Share 

0 Members and 51 Guests are viewing this topic.

LifeisaConstantStruggle

  • Forum Obsessive
  • ***
  • Posts: 307
  • Respect: +85
Re: VCE Biology Question Thread
« Reply #9285 on: August 30, 2017, 08:18:13 pm »
0
hey yall,

i need help constructing a really good Hypothesis for temperature effect on enzymes!

If anyone can help me!
Thanks xx

Hello, (as this is the hypothesis there's no need to mention enzyme denaturation or anything like that)
The higher the temperature deviation between the experimental group and the optimal temperature for the function of [insert enzyme here], the lower the rate of reaction of [substrate] completely into [product] in the presence of [enzyme]. I hope I'm not lacking information but here you go.
2016-2017: VCE (ATAR: 99.3)
2018: Monash.

KANYEWEST

  • Trailblazer
  • *
  • Posts: 36
  • Kanye for President 2020
  • Respect: +1
Re: VCE Biology Question Thread
« Reply #9286 on: August 30, 2017, 08:33:24 pm »
0
Hello, (as this is the hypothesis there's no need to mention enzyme denaturation or anything like that)
The higher the temperature deviation between the experimental group and the optimal temperature for the function of [insert enzyme here], the lower the rate of reaction of [substrate] completely into [product] in the presence of [enzyme]. I hope I'm not lacking information but here you go.
OMG THANK YOUUUUUU!
Kanye West x

simrat99

  • Trailblazer
  • *
  • Posts: 48
  • Respect: 0
Re: VCE Biology Question Thread
« Reply #9287 on: September 07, 2017, 05:35:18 pm »
0
Hi,
I was wondering, what are some of the safety precautions we need to take when handling DNA in a lab?
Thanks in advance  :)

PhoenixxFire

  • VIC MVP - 2018
  • Moderator
  • ATAR Notes Superstar
  • *****
  • Posts: 2768
  • Bad puns are how eye roll
  • Respect: +2068
Re: VCE Biology Question Thread
« Reply #9288 on: September 07, 2017, 07:07:51 pm »
0
There isn't really safety precautions specifically for DNA. You would want to be wearing gloves to prevent cross contamination. Also in the context of gel eloectrophoresis you need gloves and goggles due to ithidium bromide (a known mutagen) being used to highlight the bands.
2019: B Environment and Sustainability/B Science @ ANU

LifeisaConstantStruggle

  • Forum Obsessive
  • ***
  • Posts: 307
  • Respect: +85
Re: VCE Biology Question Thread
« Reply #9289 on: September 07, 2017, 07:46:43 pm »
0
https://www.luminexcorp.com/blog/10-ways-minimize-contamination-molecular-laboratory/

In the context of vce 5-10's probably what you need to know
2016-2017: VCE (ATAR: 99.3)
2018: Monash.

anotherworld2b

  • Forum Leader
  • ****
  • Posts: 752
  • Respect: 0
  • School Grad Year: 2017
Re: VCE Biology Question Thread
« Reply #9290 on: September 07, 2017, 11:36:02 pm »
0
I was wondering what would be a detailed explanation of how a fever works?

vox nihili

  • National Moderator
  • Great Wonder of ATAR Notes
  • *****
  • Posts: 5286
  • Respect: +1366
Re: VCE Biology Question Thread
« Reply #9291 on: September 08, 2017, 07:27:52 am »
+1

I was wondering what would be a detailed explanation of how a fever works?

NOT VCE CONTENT

I have no idea about the WACE content so I can't bill this to what you're expected to know, but:

Fevers happen when the immune system releases cytokines in response to an infectious or inflammatory stimulus. The cytokines released are TNF-α, IL-1 and IL-6. These are alternatively called pyrogens from their ability to generate (gen) heat (pyro=fire).

The pyrogens go to the hypothalamus, where they effect the production of prostaglandins (PGE2). This then leads to a change in the homeostatic set point for temperature in the hypothalamus. By changing the set point, the body starts to heat up and thus you have your fever
MED INTERVIEW TUTORING PM to secure your place early, as they fill up quickly!

Join ATARNotes Footy Tipping

2013-15: BBioMed (Biochemistry and Molecular Biology), UniMelb
2016-20: MD, UniMelb
2019: MPH, UniMelb
Year I: BIOL10002 BIOL10003 CHEM10006 MAST10011 MAST10016 PHYC10007 SPAN10001 SPAN10002
Year II: BCMB20005 BIOM20001 BIOM20002 CLAS10022 GENE20001 SPAN20020 SPAN30014
Year III: BCBM30001 BCMB30002 BCMB30010 BIOM30001 BIOM30002 PHRM30008

Primenumber32

  • Adventurer
  • *
  • Posts: 7
  • Respect: 0
Re: VCE Biology Question Thread
« Reply #9292 on: September 12, 2017, 10:15:35 pm »
0
Hey, can someone please clarify the difference between genetic testing and genetic screening.
Thanks in advance!!

vox nihili

  • National Moderator
  • Great Wonder of ATAR Notes
  • *****
  • Posts: 5286
  • Respect: +1366
Re: VCE Biology Question Thread
« Reply #9293 on: September 12, 2017, 10:46:03 pm »
+2

Hey, can someone please clarify the difference between genetic testing and genetic screening.
Thanks in advance!!

Test: I want to test you for this disease because I think you might have it
Screen: I'm gonna test a whole bunch of people and hopefully find someone with the disease
MED INTERVIEW TUTORING PM to secure your place early, as they fill up quickly!

Join ATARNotes Footy Tipping

2013-15: BBioMed (Biochemistry and Molecular Biology), UniMelb
2016-20: MD, UniMelb
2019: MPH, UniMelb
Year I: BIOL10002 BIOL10003 CHEM10006 MAST10011 MAST10016 PHYC10007 SPAN10001 SPAN10002
Year II: BCMB20005 BIOM20001 BIOM20002 CLAS10022 GENE20001 SPAN20020 SPAN30014
Year III: BCBM30001 BCMB30002 BCMB30010 BIOM30001 BIOM30002 PHRM30008

KANYEWEST

  • Trailblazer
  • *
  • Posts: 36
  • Kanye for President 2020
  • Respect: +1
Re: VCE Biology Question Thread
« Reply #9294 on: September 15, 2017, 06:09:33 pm »
0
Hey Yall, i have two quick questions on 'DNA manipulation'
1) why do we need unique nucleotides in PCR?
2) How can the Alu repeat regions (in gel electrophoresis) be used for DNA profiling?

thanks
Kanye West x

PhoenixxFire

  • VIC MVP - 2018
  • Moderator
  • ATAR Notes Superstar
  • *****
  • Posts: 2768
  • Bad puns are how eye roll
  • Respect: +2068
Re: VCE Biology Question Thread
« Reply #9295 on: September 15, 2017, 06:16:24 pm »
+1
Hey Yall, i have two quick questions on 'DNA manipulation'
1) why do we need unique nucleotides in PCR?
2) How can the Alu repeat regions (in gel electrophoresis) be used for DNA profiling?

thanks

1. Not sure what you mean by unique nucleotides. Individual A, T, C, G nucleotides have to be added, they are then joined onto the end of the primer by the taq polymerase effectively creating another strand. The taq polymerase just adds nucleotides, it does not create them so you have to put them in yourself. Does that answer your question?

2. Gel electrophoresis sorts fragments according to length. Individuals with more Alu repeats will therefore have longer fragments, which you will able to see when looking at a gel electrophoresis. If DNA from a crime scene is compared to two suspects and one suspect has a fragment of the same length as the crime scene DNA then they are likely the criminal.
2019: B Environment and Sustainability/B Science @ ANU

KANYEWEST

  • Trailblazer
  • *
  • Posts: 36
  • Kanye for President 2020
  • Respect: +1
Re: VCE Biology Question Thread
« Reply #9296 on: September 15, 2017, 09:33:57 pm »
0
1. Not sure what you mean by unique nucleotides. Individual A, T, C, G nucleotides have to be added, they are then joined onto the end of the primer by the taq polymerase effectively creating another strand. The taq polymerase just adds nucleotides, it does not create them so you have to put them in yourself. Does that answer your question?

2. Gel electrophoresis sorts fragments according to length. Individuals with more Alu repeats will therefore have longer fragments, which you will able to see when looking at a gel electrophoresis. If DNA from a crime scene is compared to two suspects and one suspect has a fragment of the same length as the crime scene DNA then they are likely the criminal.

Thank you so much for your reply,
It makes sense now!
:)
Kanye West x

KANYEWEST

  • Trailblazer
  • *
  • Posts: 36
  • Kanye for President 2020
  • Respect: +1
Re: VCE Biology Question Thread
« Reply #9297 on: September 15, 2017, 10:21:41 pm »
0
Thank you so much for your reply,
It makes sense now!
:)

Also, to elaborate more on the first question, the question simply stated:
'The PCR primers used in this experiment are unique nucleotide sequences. why would they need to be unique sequences?'

Im not to sure on how to answer this question, as my teacher doesnt like basic answers, so i wanted to ask for help from here, which you did help me with, but now that i have included the entire question, would you be able to answer it for me, or is it jsut what you mentioned on earlier?
Kanye West x

PhoenixxFire

  • VIC MVP - 2018
  • Moderator
  • ATAR Notes Superstar
  • *****
  • Posts: 2768
  • Bad puns are how eye roll
  • Respect: +2068
Re: VCE Biology Question Thread
« Reply #9298 on: September 15, 2017, 10:40:44 pm »
0
ahhh ok that makes more sense. It's a weirdly worded question I doubt you would get one like that from VCAA. I'd say the answer they're looking for would be something like

PCR primers need to be able to anneal to either side of the section of DNA that is intended to be amplified. To do this they must be complementary to the unique sequence of DNA on either side of the desired region.

ie. They have to be unique so they join to the right spot (the complementary DNA sequence)
2019: B Environment and Sustainability/B Science @ ANU

KANYEWEST

  • Trailblazer
  • *
  • Posts: 36
  • Kanye for President 2020
  • Respect: +1
Re: VCE Biology Question Thread
« Reply #9299 on: September 15, 2017, 10:53:12 pm »
0
ahhh ok that makes more sense. It's a weirdly worded question I doubt you would get one like that from VCAA. I'd say the answer they're looking for would be something like

PCR primers need to be able to anneal to either side of the section of DNA that is intended to be amplified. To do this they must be complementary to the unique sequence of DNA on either side of the desired region.

ie. They have to be unique so they join to the right spot (the complementary DNA sequence)

That sounds excellent!
Thank you again for your help, hugely appriciated!
Kanye West x