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October 16, 2019, 08:35:28 pm

Author Topic: VCE Biology Question Thread  (Read 1378577 times)  Share 

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RazzMeTazz

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Re: VCE Biology Question Thread
« Reply #2610 on: August 16, 2014, 10:42:26 am »
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What's the difference between 'recombinant DNA' and a 'transgene'?

Reus

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Re: VCE Biology Question Thread
« Reply #2611 on: August 16, 2014, 06:38:44 pm »
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What's the difference between 'recombinant DNA' and a 'transgene'?
Well the difference between the two is that, transgene is a gene that is not native/belonging to an organism (a gene that is transferred from one organism to another) whereas recombinant DNA is DNA that has essentially been combined with other, different DNAs (manipulated DNA - contains more the one organisms' DNA).

An easy way of remembering it is that DNA is double-stranded right? So during recombination, the strands are unzipped into two, thus allowing another strand to bind with either of the original strands (aka. a transgene) resulting in recombinant DNA.
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Reus

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Re: VCE Biology Question Thread
« Reply #2612 on: August 16, 2014, 06:39:58 pm »
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What types of environments support the evolutionary change of punctuated equilibrium and gradualism?
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vox nihili

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Re: VCE Biology Question Thread
« Reply #2613 on: August 16, 2014, 07:06:05 pm »
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What types of environments support the evolutionary change of punctuated equilibrium and gradualism?

Not the VCE environment. This isn't part of the course.
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RazzMeTazz

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Re: VCE Biology Question Thread
« Reply #2614 on: August 17, 2014, 09:20:22 am »
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In the process of DNA profiling how is the DNA cut to produce just the fragments of STRs?

Also would it be correct to assume that reverse transcriptase is used to obtain the DNA sequence of a gene of interest, as mRNA is easier to obtain from a cell, than nuclear DNA?
« Last Edit: August 17, 2014, 12:38:36 pm by RazzMeTazz »

Jason12

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Re: VCE Biology Question Thread
« Reply #2615 on: August 17, 2014, 02:17:31 pm »
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do we need to know a lot about induction/repression?
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nerdmmb

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Re: VCE Biology Question Thread
« Reply #2616 on: August 17, 2014, 03:16:51 pm »
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What exactly is a buffer that is used in gel electrophoresis?

Thanks!

RazzMeTazz

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Re: VCE Biology Question Thread
« Reply #2617 on: August 17, 2014, 03:28:17 pm »
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Is DNA profiling always done using the hypervariable regions of DNA?

InsaneMDot

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Re: VCE Biology Question Thread
« Reply #2618 on: August 17, 2014, 04:58:33 pm »
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What exactly is a buffer that is used in gel electrophoresis?

Thanks!

The buffer is just a solution which provides the environment for DNA polymerase, helping it to function

nerdmmb

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Re: VCE Biology Question Thread
« Reply #2619 on: August 17, 2014, 05:19:43 pm »
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Not sure if I've asked this before but is there a difference between DNA profiling and DNA fingerprinting?

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Scooby

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Re: VCE Biology Question Thread
« Reply #2620 on: August 17, 2014, 05:39:25 pm »
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The buffer is just a solution which provides the environment for DNA polymerase, helping it to function

We use a DNA polymerase (Taq polymerase) for PCR, but not gel electrophoresis. The buffer allows the current to be carried and helps to stabilise the pH as well
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dankfrank420

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Re: VCE Biology Question Thread
« Reply #2621 on: August 19, 2014, 07:32:27 pm »
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I'm a bit confused regarding the relationship between genes and alleles.

I know that alleles are alternative forms of a gene. However, if someone is heterozygous for eye colour, does that mean that he has different genes to someone that is homozygous for eye colour? Wouldn't that person have the same genes (eg. eye colour) but have different alleles (eg. Bb and BB)?

grannysmith

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Re: VCE Biology Question Thread
« Reply #2622 on: August 19, 2014, 07:41:46 pm »
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Eye colour is the gene. It's the gene locus and within that gene locus there are different forms of the gene i.e. alleles.

Yes, you're correct. They have the same gene, but different FORMS of the gene.
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RazzMeTazz

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Re: VCE Biology Question Thread
« Reply #2623 on: August 19, 2014, 07:47:17 pm »
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Do all organisms have STRs?

What about VNTRs?

Also, is this correct: All individuals have the same STRs but the repetition of these STRs varies between individuals.

For example: On chromosome 1, between gene 1 and 2 I may have the STR of CA, which is repeated four times: CACACACA

and on chromosome 1 between gene 1 and 2 for all other individuals, the STR of CA would also be present, but repeated a different number of times?

RazzMeTazz

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Re: VCE Biology Question Thread
« Reply #2624 on: August 19, 2014, 08:22:09 pm »
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What are the hypervariable regions in DNA?

Are they related to STRs?