Hey ATARNotes,
I promise I will not be learning all of Unit 3 or do anything crazy, I am just trying to reduce the workload for when I get bombarded with work in the first 2 weeks of school. I am mostly aware of the importance of taking a break. If there are questions that you think are silly, or could be easily googled, please call me out for it. A lot of this is just for determining how much I need to know and get a feel for what's required.
Does translation occur inside the rER? I believe that only protein modification would occur in the rough endoplasmic reticulum, given that there are ribosomes that would be translating the mRNA strand into amino acids on the exterior surface of the rER.
Thus, would we be required to say that translation occurs in the cytosol, as saying cytoplasm would then also include inside organelles? I understand Google says that it would occur in the cytoplasm, but, if it does not occur inside the rER, would this be incorrect, given that translation does not occur in any other organelle?
Also:
2. What exactly would tertiary structure be? Is it just the overall 3D structure of the protein, i.e going beyond just the secondary structure, but now including the 'deliberate' folds of the protein made in the rER? This seems really confusing as various sources are saying that the tertiary structure is the 3D shape- yet even the molecules that make up the primary and secondary structure (which is just if there are alpha helices and beta pleated sheets present) are 3 dimensional.
3. With DNA and RNA, is it sufficient to know the similarities and differences, what they are made up of, their condensation polymerisation reactions and that the RNA polymerase binds to the promoter region at the 3' to start reading the DNA template strand? How granular do we need to get regarding the structure of these nucleic acids? (Do we need to know about the diagrammatic representation of nucleosomes to chromosomes and is it required that we understand what distinguishes uracil from thymine? Is this too much?)
4. What might we be required to know about the upstream/downstream regions- is knowing that they do not include the stop and start codons, if mutated can change the structure of the protein fine?
Sorry for asking so many questions