I never got my head around dilution, so can someone clarify? :/
So I have a stock standard solution: 1000mg/100mL of caffeine
I want to make concentrations of 5mg/100ml, 10mg/100ml and 20mg/100ml caffeine.
Say for the first concentration I want to obtain (5mg/100ml), do i simply get like 0.5mL of the standard (5mg/0.5mL), and then dilute it into 100mL?
Is that how it works? o.o
Or is my logic wrong. hahhaha
Thanks!
So initially, you have 1000mg/100mL, then after dilution, you have 5 mg/100 mL, you can see that 1000/5 = 200 . If concentration decrease by 200 folds, volume must have increased by 200 folds. Hence, 100 x 200 = 20 000 mL i.e if you fill the volume up to 20 00mL (adding 19 900 mL), you get a concentration of 5 mg/100mL.
Does my method still work? o.o