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March 29, 2024, 09:58:12 am

Author Topic: QUICK CRISPR QUESTION  (Read 3580 times)  Share 

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I.09

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QUICK CRISPR QUESTION
« on: April 27, 2021, 08:10:15 pm »
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Hey Guys,

I have been stuck on this for ages..
so when the biotechnology of CRISPR is used to cut the double-stranded DNA of the cell and a new gene is inserted, where does this new gene come from? Does it come from another organism or is it synthesised by humans?

I am so confused, any help will be greatly appreciated.
Thank you so much.

sweetiepi

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Re: QUICK CRISPR QUESTION
« Reply #1 on: April 27, 2021, 09:09:34 pm »
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A new gene can come from insertion of a template gene via the homology directed repair pathway, or random bases fill in where the Cas protein cuts via the Non-Homolgous End Joining pathway. (The former is called Knock-in, and the latter often causes a gene knock-out)

These pathways are competitive against each other, and NHEJ is more commonly observed compared to HDR.

Furthermore, there are several other ways to insert a gene or change a base with CRISPR/Cas9 such as base editing and prime base editing but that may be out of your syllabus scope :)
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) Read my uni journey here!

I.09

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Re: QUICK CRISPR QUESTION
« Reply #2 on: April 27, 2021, 10:18:40 pm »
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Thank you so much!!
So, those are the two ways in which genes can be inserted via CRISPR, but is the gene itself from another organism or lab-made?

sweetiepi

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Re: QUICK CRISPR QUESTION
« Reply #3 on: April 27, 2021, 11:00:56 pm »
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Thank you so much!!
So, those are the two ways in which genes can be inserted via CRISPR, but is the gene itself from another organism or lab-made?
It can be from the organism itself (for bases) or as an external template (I.e. lab-made or from other gene sources)
2017-2019: Bachelor of Pharmaceutical Science (Formulation Science)
2020: Bachelor of Pharmaceutical Science (Honours) Read my uni journey here!